Development of Multiplex qPCR Assay for Periodontal Bacteria Detection
Contributors:
Josh Coffey, BS1
Mydah Choudhry, BA2
Vineet Singh, Phd2
Josh Coffey, BS1
Mydah Choudhry, BA2
Vineet Singh, Phd2
Participating Organizations:
Missouri School of Dentistry and Oral Health
Kirksville College of Osteopathic Medicine
Missouri School of Dentistry and Oral Health
Kirksville College of Osteopathic Medicine
Background
The development and severity of periodontitis is strongly associated with the type and quantity of subgingival bacteria. The current paradigm for evaluating periodontal disease is through clinical measurements: probing depths, attachment loss, BOP, etc. Clinicians are not currently measuring bacterial load, despite evidence that the etiology and prognosis of periodontal disease is microbially driven. Different techniques have been used for examining microbial composition, but each have drawbacks including time, accuracy, cost, and ability to quantify small samples. Through multiplex real time qPCR it is possible to accurately detect and quantify up to 5 different pathogens in a single assay, saving time and money. The development of such an assay would be a valuable tool for translational research.
The development and severity of periodontitis is strongly associated with the type and quantity of subgingival bacteria. The current paradigm for evaluating periodontal disease is through clinical measurements: probing depths, attachment loss, BOP, etc. Clinicians are not currently measuring bacterial load, despite evidence that the etiology and prognosis of periodontal disease is microbially driven. Different techniques have been used for examining microbial composition, but each have drawbacks including time, accuracy, cost, and ability to quantify small samples. Through multiplex real time qPCR it is possible to accurately detect and quantify up to 5 different pathogens in a single assay, saving time and money. The development of such an assay would be a valuable tool for translational research.
